Catechol Oxidase Lab

Catechol oxidase is an enzyme that speeds up the oxidation reaction when catechol is uncovered to oxygen. When the reaction occurs, quin oneness is produced go the oxidized content brown. It was hypothesized that the taller the constriction of catechol oxidase, the browner the magnetic core will turn, and the faster it will gain the ruse. In the present lab, several(predicate) meannesss of catechol oxidase were mixed with stark(a) catechol and the rate at which each issue browned was thrifty employ a colorimeter.The results showed that the ascendent with the spicy concentration of catechol oxidase had the fas judge rate at which it turned brown. However, it did non turn the brownest, the solution with the strong suit concentration of enzyme did. These results show that our hypothesis was twain supported and not supported and it was concluded that increase in catechol oxidase concentration increases rate of turn of events brown but does not increase the take of br ownness a substance becomes.IntroductionHave you ever wondered why the inside of your orchard apple tree turns brown if you leave it on the counter after taking a bite? This is because there is a compound within the apple called catechol which, when exposed to the air, becomes oxidized by the oxygen in the air and produces benzoquinone, which makes the apple turn up brown (Andrew Clappers Online Journal, 2007). This chemical reaction is catalyzed by the enzyme catechol oxidase (Figure A.1). Enzymes argon proteins in cells that speed up chemical reactions (Enzyme Procedure Guide, 2013). The catechol oxidase lowers the amount of cleverness needed in order for the catechol to be oxidized, which speeds the mould up.Fig. A.1 The oxidation process of catechol with the help of catechol oxidase produces benzoquinone. (Enzyme Procedure PowerPoint, 2013)If you frequently eat apples you may have as well noticed that different types apples brown at different rates. This is because each fle sh of apple has a different concentration of catechol oxidase (Daniela Finkel, 2013). Therefore, each apple has a unique speed at which it will produce benzoquinone, and turn brown.This investigate was designed to investigate the following hypothesis The eminenter the concentration of catechol oxidase, the browner the substance will be, and the faster it will achieve the color (Enzyme Procedure Guide, 2013).Within the experiment, excellent catechol was mixed with different concentrations of catechol oxidase and the rate at which each solution produced benzoquinone was measured. The amount of benzoquinone made throughout the trials was measured by using a colorimeter to measure the level of brownness of the liquid. The colorimeter worked by shining a fainthearted through the liquid and then amount that light on the other side to see how much of it was absorbed.In this experiment, absorbance of inexorable light was measured because unsanctified light is absorbed by the color br own. The amount of blue light absorbance was measured every(prenominal) 15 seconds for pentad minutes. Because enzymes speed up reactions, more enzymes would cause the reaction to be point faster.1Procedure/Materials and MethodsThe lab leaders and the Punk Rock Warlord prepared three different concentrations of catechol oxidase by extracting potato juice (because it contains lots of catechol oxidase). Pure catechol, a 5mL test tube, 1mL/5mL syringes, a colorimeter and water were also used. 4mL of pure catechol and 1mL of the highly knockout catechol oxidase were measured with the syringes. Both liquids were then injected into the 5mL test tube. The 5mL tube was placed into the spring in the colorimeter and the lid of thecolorimeter was closed.The measure of absorbance of blue light displayed on the screen of the colorimeter was recorded every 15 seconds for atomic number 23 minutes. This procedure was repeated for two more trials, each with 4mL of pure catechol one used 1mL of medium punishing catechol oxidase and one used 1mL of low concentrated catechol oxidase.The independent variable was the concentration of catechol oxidase, the dependent variable was the rate of turning brown, there were no controls, there were three replications and the sample sizes were 11 (for high concentration) and 10 (for medium and low concentrations).Results SectionFigure 1 shows that before the rates began to plateau, the high enzyme concentration solution was turning brown the fastest. Table 1 shows that the high enzyme concentration had the fastest rate and that on average, it deviated from the regard as more than the medium and low concentrations. Figure 2 shows that the high enzyme concentration solution was turning brown the fastest. Table 2 shows that the different rates each solution turned brown are statistically significant.Table 1 Average place of for High, Medium and humiliated Concentrations of Catechol Oxidase and the Absolute Average Deviations. iii diff erent concentrations of catechol oxidase were added to pure catechol and the rate of change in the absorption of blue light (production of benzoquinone) was measured every 15 seconds using a colorimeter. Data shown as mean of % of absorption per minute. N=11 for the high concentration and n=10 for the medium and low concentrations. Concentration of CatecholAverage Slope (Rate) Abs. Ave. Dev. High 0.818 0.174 Medium 0.569 0.081 Low 0.196 0.120Table 2 T-Test Results When Comparing the Rates at Which Different Solutions Browned Three different concentrations of catechol oxidase were added to pure catechol and the rate of change in the absorption of blue light (because production of benzoquinone makes a substance brown) was measured every 15 seconds using a colorimeter. N=11 for high concentration and n=10 for medium and low concentrations. Enzyme ConcentrationsP-Value Low vs. Medium